High-resolution melting assay for genotyping of IFNL4-associated dinucleotide variant rs368234815
نویسندگان
چکیده
منابع مشابه
Multiplex amplicon genotyping by high-resolution melting.
High-resolution amplicon melting is a simple method for genotyping that uses only generic PCR primers and a saturating DNA dye. Multiplex amplicon genotyping has previously been reported in a single color, but two instruments were required: a carousel-based rapid cycler and a high-resolution melting instrument for capillaries. Manual transfer of capillaries between instruments and sequential me...
متن کاملGenotyping accuracy of high-resolution DNA melting instruments.
BACKGROUND High-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assessed. METHODS Each genotype of the single nucleotide variant (SNV) (c.3405-29A>T) of CP...
متن کاملHigh-resolution genotyping by amplicon melting analysis using LCGreen.
BACKGROUND High-resolution amplicon melting analysis was recently introduced as a closed-tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003;49:396-406). The technique required a fluorescently labeled primer and was limited to the detection of mutations residing in the melting domain of the labeled primer. Our aim was to develop a closed-tube system for genotyping and...
متن کاملAnalysis of HIV diversity using a high-resolution melting assay.
HIV viruses are usually genetically homogeneous shortly after infection, and become more heterogeneous over time. We developed a high-resolution melting (HRM) assay to analyze HIV diversity without sequencing. Plasma samples from the HIVNET 012 trial were obtained from nine Ugandan mother-infant pairs. DNA amplified from the HIV gag region was analyzed to determine the number of degrees over wh...
متن کاملDevelopment of a High-Resolution Melting Method for Screening R188H Polymorphism in XRCC2 Gene
Background: The High Resolution Melting (HRM) method is a new scanning method for detecting unknown changes in DNA and its advantages have persuaded researchers to recruit it as a screening method. Objectives: Here, we developed a HRM method to screen R188H SNP (rs3218536) of XRCC2 and compared the results with a well known PCR-RFLP technique. Materials and Methods: Genomic ...
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ژورنال
عنوان ژورنال: Clinical Microbiology and Infection
سال: 2014
ISSN: 1198-743X
DOI: 10.1111/1469-0691.12637